As of today, Amazon.com is stocking a book to which I have contributed, RNA-seq Data Analysis: A Practical Approach. I realize the title might sound obscure to readers who are unfamiliar with genomics and bioinformatics. Simply put, RNA-seq is short for RNA sequencing, a method for measuring what we call gene expression. While the DNA contained in each cell is (to a first approximation) identical, different tissues and cell types turn their genes on and off in different ways in response to different conditions. The process when DNA is transcribed to RNA is called gene expression. RNA-seq has become a rather important experimental method and the lead author of our book, Eija Korpelainen, wanted to put together a user-friendly, practical and hopefully unbiased compendium of the existing RNA-seq data analysis methods and toolkits, without neglecting underlying theory. I contributed one chapter, the one about differential expression analysis, which basically means statistical testing for significant gene expression differences between groups of samples.
I am also currently involved as an assistant teacher in the Explore Statistics with R course given by Karolinska Institutet through the edX MOOC platform. Specifically, I have contributed material to the final week (week 5) which will start next Tuesday (October 7th). That material is also about RNA-seq analysis – I try to show a range of tools available in R which allow you to perform a complete analysis workflow for a typical scenario. Until the fifth week starts, I am helping out with answering student questions in the forums. It’s been a positive experience so far, but it is clear that one can never prepare enough for a MOOC – errors in phrasing, grading, etc are bound to pop up. Luckily, several gifted students are doing an amazing job of answering the questions from other students, while teaching us teachers a thing or two about the finer points of R.
Speaking of MOOCs, Coursera’s Mining Massive Datasets course featuring Jure Leskovec, Anand Rajaraman and Jeff Ullman started today. My plan is to try to follow it – we shall see if I have time.